DEPRECATED - Run the fastq-multx program from ea-utils to demultiplex a reads library into reads sets.
Deprecation Announcement:
This App is being deprecated due to a high fail rate: meeting the criteria for deactivation.
Demultiplex with ea-utils FASTQ-MULTX is in the process of being refactored and improved by the Kbase Team. If this app is needed for your work or analyses in KBase please contact our team.
This App demultiplexes a reads library into a ReadsSet using the FASTQ-MULTX program in the EA Utils package. It is capable of automatically determining barcode IDs, and can verify that multiple reads are kept in-sync during demultiplexing.
Team members who developed & deployed algorithm in KBase: Dylan Chivian.
Related Publications
- Arkin AP, Cottingham RW, Henry CS, Harris NL, Stevens RL, Maslov S, et al. KBase: The United States Department of Energy Systems Biology Knowledgebase. Nature Biotechnology. 2018;36: 566. doi: 10.1038/nbt.4163 , https://www.nature.com/articles/nbt.4163
- EA Utils package source: , https://expressionanalysis.github.io/ea-utils/
App Specification:
https://github.com/kbaseapps/kb_ea_utils/tree/b09edaab7d1102890e8ff1dde33f142de38a64e6/ui/narrative/methods/run_Fastq_MultxModule Commit: b09edaab7d1102890e8ff1dde33f142de38a64e6