FastQCFastQC Report
Tue 27 Apr 2021
23_reads_51640_4_1.fwd.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename23_reads_51640_4_1.fwd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916113
Sequences flagged as poor quality0
Sequence length35-151
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGG2950.00334598732.7566145
TAGCCCG950.007207111528.0035325
GGGGGGG9758.899062E-619.821941145
TAGACTG4855.4569682E-1119.1982985
ACCGATC2851.4956957E-518.6690228
CTAGGAC2400.001889474316.6270963
GACCGTC2550.002832175715.6490337
AGACAGG6550.001587001714.752972145
GTCTACG5005.567763E-714.6322291
TATACAG21150.014.1507225
GTATTAG36300.014.1082091
CCGTACA2850.00593320814.0014019
TATACTG21250.013.7711495
GTATATA39950.013.4851571
GACAGTC3952.4338422E-413.4700547
TACACCG6454.9476512E-813.4047915
GTCTTAC10550.013.2389821
CCGTGTA3550.001667614313.1139889
GACTGTG5653.1360534E-512.827251145
GTATAAT37100.012.72835351
Produced by FastQC (version 0.11.5)