FastQCFastQC Report
Mon 13 Jul 2020
Hydraulic_fracture_well_fluid_raw_reads_67335_4_1.fwd.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHydraulic_fracture_well_fluid_raw_reads_67335_4_1.fwd.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42872661
Sequences flagged as poor quality0
Sequence length108
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT111600.08.546285102
ACACGTC119750.08.092413102
GCACACG149350.07.6155205102
CACGTCT124050.07.318518102
AGCACAC193450.06.7231283102
GATCGGA363150.06.502718102
TCGGAAG317850.06.29019102
ACGTCTG120550.05.9655685102
TAGATCG173950.05.9227977102
AGATCGG447200.05.7367525102
GAGCACA243000.05.688044102
AGAGCAC294850.05.5353975102
ATCGGAA406250.05.473852102
CGGAAGA385900.05.405657102
CGTCTGA125400.05.0840807102
Produced by FastQC (version 0.11.5)