Generated July 18, 2024
from biokbase.narrative.jobs.appmanager import AppManager
AppManager().run_app_batch(
    [{
        "app_id": "kb_uploadmethods/import_fastq_noninterleaved_as_reads_from_staging",
        "tag": "release",
        "version": "5b9346463df88a422ff5d4f4cba421679f63c73f",
        "params": [{
            "fastq_fwd_staging_file_name": "SQK-RBK114-24_barcode07.fastq",
            "fastq_rev_staging_file_name": None,
            "name": "colony27_barcode07"
        }],
        "shared_params": {
            "sequencing_tech": "NanoPore",
            "single_genome": 1,
            "read_orientation_outward": 0,
            "insert_size_std_dev": None,
            "insert_size_mean": None
        }
    }],
    cell_id="8441dd38-7af5-4591-aec1-2ae30b523b90",
    run_id="1a4c0972-77b0-40d4-8fd2-46e1d08b6042"
)
Filter long reads using Filtlong.
This app completed without errors in 1m 24s.
Objects
Created Object Name Type Description
colony27_barcode07_filtlong.reads SingleEndLibrary Filtered reads
Summary
Getting long reads (from reads library object). command: filtlong --min_length 1000 --keep_percent 90 --target_bases 500000000 /kb/module/work/tmp/9949cdcd-92d9-41c0-9b5e-352c3c0531f8.single.fastq > /kb/module/work/tmp/filtlong_output_e1147e3c-133e-44b3-ad59-0d1c96709e3c.fastq Scoring long reads 72 reads (500871 bp) 159 reads (994324 bp) 236 reads (1491593 bp) 322 reads (1978751 bp) 406 reads (2485949 bp) 496 reads (2975627 bp) 582 reads (3461582 bp) 673 reads (3946351 bp) 754 reads (4433068 bp) 830 reads (4921079 bp) 915 reads (5406509 bp) 1011 reads (5890517 bp) 1101 reads (6389406 bp) 1181 reads (6878874 bp) 1270 reads (7365987 bp) 1339 reads (7851145 bp) 1418 reads (8352604 bp) 1512 reads (8836986 bp) 1615 reads (9325342 bp) 1709 reads (9834183 bp) 1847 reads (10338321 bp) 1922 reads (10822492 bp) 2036 reads (11329966 bp) 2163 reads (11817488 bp) 2232 reads (12308054 bp) 2319 reads (12817348 bp) 2402 reads (13306384 bp) 2507 reads (13790001 bp) 2597 reads (14276564 bp) 2666 reads (14763111 bp) 2754 reads (15249069 bp) 2840 reads (15743075 bp) 2924 reads (16235121 bp) 3004 reads (16747292 bp) 3097 reads (17237195 bp) 3174 reads (17725118 bp) 3268 reads (18219416 bp) 3361 reads (18710621 bp) 3439 reads (19206588 bp) 3540 reads (19691258 bp) 3637 reads (20179056 bp) 3709 reads (20666750 bp) 3851 reads (21153612 bp) 4001 reads (21641629 bp) 4091 reads (22126858 bp) 4176 reads (22618090 bp) 4280 reads (23107327 bp) 4370 reads (23597629 bp) 4444 reads (24104606 bp) 4516 reads (24588289 bp) 4599 reads (25080954 bp) 4675 reads (25566740 bp) 4751 reads (26057607 bp) 4825 reads (26543489 bp) 4917 reads (27033620 bp) 4997 reads (27521892 bp) 5081 reads (28006281 bp) 5157 reads (28496154 bp) 5243 reads (28983530 bp) 5320 reads (29467690 bp) 5402 reads (29952732 bp) 5506 reads (30451572 bp) 5589 reads (30936187 bp) 5674 reads (31428210 bp) 5777 reads (31915112 bp) 5878 reads (32404661 bp) 5957 reads (32894100 bp) 6129 reads (33388517 bp) 6240 reads (33874219 bp) 6317 reads (34359994 bp) 6424 reads (34856652 bp) 6523 reads (35350584 bp) 6606 reads (35837477 bp) 6697 reads (36357360 bp) 6785 reads (36847653 bp) 6894 reads (37332279 bp) 6986 reads (37823053 bp) 7060 reads (38309983 bp) 7136 reads (38802017 bp) 7208 reads (39291243 bp) 7310 reads (39781875 bp) 7382 reads (40275420 bp) 7486 reads (40759752 bp) 7576 reads (41245421 bp) 7655 reads (41739669 bp) 7755 reads (42231424 bp) 7817 reads (42723998 bp) 7906 reads (43207980 bp) 8000 reads (43692529 bp) 8116 reads (44178950 bp) 8229 reads (44665601 bp) 8338 reads (45150677 bp) 8430 reads (45637469 bp) 8523 reads (46121820 bp) 8596 reads (46610264 bp) 8676 reads (47096399 bp) 8765 reads (47585888 bp) 8843 reads (48079879 bp) 8918 reads (48570765 bp) 8974 reads (49072003 bp) 9048 reads (49559943 bp) 9137 reads (50044582 bp) 9217 reads (50541261 bp) 9321 reads (51035479 bp) 9422 reads (51524693 bp) 9491 reads (52012532 bp) 9586 reads (52497464 bp) 9663 reads (53008089 bp) 9743 reads (53492079 bp) 9821 reads (53976165 bp) 9902 reads (54460322 bp) 10001 reads (54944250 bp) 10113 reads (55433372 bp) 10189 reads (55921054 bp) 10288 reads (56412533 bp) 10392 reads (56901614 bp) 10530 reads (57390791 bp) 10629 reads (57875190 bp) 10730 reads (58359267 bp) 10815 reads (58845915 bp) 10911 reads (59330709 bp) 10990 reads (59817934 bp) 11063 reads (60307268 bp) 11156 reads (60797605 bp) 11235 reads (61284275 bp) 11328 reads (61778564 bp) 11390 reads (62264192 bp) 11470 reads (62749383 bp) 11545 reads (63233789 bp) 11638 reads (63721003 bp) 11734 reads (64205507 bp) 11810 reads (64694366 bp) 11914 reads (65185649 bp) 12015 reads (65670084 bp) 12123 reads (66160451 bp) 12226 reads (66648872 bp) 12342 reads (67132967 bp) 12475 reads (67621865 bp) 12568 reads (68110026 bp) 12643 reads (68594988 bp) 12734 reads (69081220 bp) 12802 reads (69571116 bp) 12899 reads (70054885 bp) 12975 reads (70541134 bp) 13050 reads (71028905 bp) 13129 reads (71517137 bp) 13212 reads (72001630 bp) 13274 reads (72490117 bp) 13360 reads (72973797 bp) 13442 reads (73457725 bp) 13511 reads (73944551 bp) 13565 reads (74440459 bp) 13652 reads (74926039 bp) 13731 reads (75412050 bp) 13810 reads (75907615 bp) 13903 reads (76395412 bp) 13994 reads (76881249 bp) 14114 reads (77368561 bp) 14209 reads (77852810 bp) 14309 reads (78339751 bp) 14399 reads (78824624 bp) 14487 reads (79311265 bp) 14596 reads (79801580 bp) 14679 reads (80289195 bp) 14762 reads (80773458 bp) 14876 reads (81274759 bp) 14971 reads (81765286 bp) 15045 reads (82258954 bp) 15163 reads (82746353 bp) 15263 reads (83231458 bp) 15356 reads (83717673 bp) 15446 reads (84209730 bp) 15521 reads (84694611 bp) 15610 reads (85179267 bp) 15695 reads (85663757 bp) 15777 reads (86149665 bp) 15863 reads (86637443 bp) 15933 reads (87123340 bp) 16037 reads (87609347 bp) 16171 reads (88095879 bp) 16266 reads (88581957 bp) 16329 reads (89079752 bp) 16415 reads (89566835 bp) 16488 reads (90053549 bp) 16593 reads (90546156 bp) 16685 reads (91030580 bp) 16790 reads (91520403 bp) 16886 reads (92007165 bp) 16957 reads (92493905 bp) 17073 reads (92979382 bp) 17181 reads (93463744 bp) 17252 reads (93947889 bp) 17349 reads (94434258 bp) 17444 reads (94926732 bp) 17546 reads (95411084 bp) 17632 reads (95899238 bp) 17730 reads (96388300 bp) 17815 reads (96876099 bp) 17888 reads (97359920 bp) 17981 reads (97857202 bp) 18088 reads (98341395 bp) 18199 reads (98846225 bp) 18279 reads (99331797 bp) 18357 reads (99815859 bp) 18436 reads (100304614 bp) 18524 reads (100791268 bp) 18618 reads (101281531 bp) 18726 reads (101765976 bp) 18826 reads (102260496 bp) 18904 reads (102772790 bp) 18986 reads (103264520 bp) 19113 reads (103750253 bp) 19201 reads (104242321 bp) 19310 reads (104736062 bp) 19396 reads (105222956 bp) 19474 reads (105722972 bp) 19621 reads (106209394 bp) 19690 reads (106694252 bp) 19786 reads (107179422 bp) 19894 reads (107665130 bp) 19991 reads (108148870 bp) 20078 reads (108633159 bp) 20188 reads (109125413 bp) 20274 reads (109613774 bp) 20401 reads (110110893 bp) 20493 reads (110601287 bp) 20606 reads (111093851 bp) 20677 reads (111578534 bp) 20762 reads (112063815 bp) 20853 reads (112552800 bp) 20956 reads (113040378 bp) 21037 reads (113533019 bp) 21121 reads (114032905 bp) 21196 reads (114520636 bp) 21335 reads (115005031 bp) 21407 reads (115496583 bp) 21480 reads (115981634 bp) 21597 reads (116476748 bp) 21685 reads (116963351 bp) 21768 reads (117447454 bp) 21861 reads (117965015 bp) 21948 reads (118457052 bp) 22046 reads (118943531 bp) 22121 reads (119439053 bp) 22243 reads (119950657 bp) 22356 reads (120434347 bp) 22435 reads (120919298 bp) 22526 reads (121405621 bp) 22594 reads (121900939 bp) 22685 reads (122393338 bp) 22768 reads (122878195 bp) 22849 reads (123363764 bp) 22919 reads (123847844 bp) 23013 reads (124336252 bp) 23095 reads (124824261 bp) 23192 reads (125308171 bp) 23288 reads (125795690 bp) 23386 reads (126292896 bp) 23520 reads (126788202 bp) 23601 reads (127278472 bp) 23688 reads (127763524 bp) 23762 reads (128249825 bp) 23861 reads (128742870 bp) 23944 reads (129237713 bp) 24034 reads (129729306 bp) 24124 reads (130216971 bp) 24194 reads (130702130 bp) 24299 reads (131186603 bp) 24392 reads (131673332 bp) 24500 reads (132158882 bp) 24604 reads (132648485 bp) 24701 reads (133132674 bp) 24808 reads (133617597 bp) 24894 reads (134105799 bp) 24979 reads (134595027 bp) 25067 reads (135086577 bp) 25164 reads (135575921 bp) 25240 reads (136074597 bp) 25346 reads (136558517 bp) 25441 reads (137053589 bp) 25508 reads (137538844 bp) 25599 reads (138034534 bp) 25710 reads (138535403 bp) 25788 reads (139027540 bp) 25890 reads (139511420 bp) 25981 reads (139996525 bp) 26070 reads (140496220 bp) 26178 reads (140982089 bp) 26245 reads (141471946 bp) 26327 reads (141959943 bp) 26408 reads (142444501 bp) 26513 reads (142928472 bp) 26622 reads (143419252 bp) 26730 reads (143906784 bp) 26805 reads (144411975 bp) 26893 reads (144897538 bp) 26963 reads (145403101 bp) 27048 reads (145894655 bp) 27140 reads (146385589 bp) 27226 reads (146874864 bp) 27322 reads (147361420 bp) 27406 reads (147876653 bp) 27518 reads (148370432 bp) 27641 reads (148872301 bp) 27722 reads (149356930 bp) 27818 reads (149848759 bp) 27938 reads (150332994 bp) 28042 reads (150819797 bp) 28143 reads (151319299 bp) 28232 reads (151809847 bp) 28326 reads (152298814 bp) 28413 reads (152784753 bp) 28522 reads (153276248 bp) 28628 reads (153764409 bp) 28732 reads (154250871 bp) 28835 reads (154742850 bp) 28922 reads (155242468 bp) 29016 reads (155728936 bp) 29127 reads (156212651 bp) 29206 reads (156696678 bp) 29295 reads (157191001 bp) 29379 reads (157676972 bp) 29457 reads (158162749 bp) 29577 reads (158661283 bp) 29673 reads (159148174 bp) 29759 reads (159637288 bp) 29838 reads (160122343 bp) 29928 reads (160612751 bp) 30055 reads (161099092 bp) 30128 reads (161586332 bp) 30239 reads (162093486 bp) 30314 reads (162581070 bp) 30404 reads (163071526 bp) 30504 reads (163555862 bp) 30604 reads (164071344 bp) 30675 reads (164562725 bp) 30756 reads (165047156 bp) 30844 reads (165531670 bp) 30942 reads (166041673 bp) 31011 reads (166538704 bp) 31092 reads (167031024 bp) 31170 reads (167529448 bp) 31243 reads (168019979 bp) 31338 reads (168512205 bp) 31448 reads (169001440 bp) 31528 reads (169491956 bp) 31610 reads (169978613 bp) 31697 reads (170481917 bp) 31812 reads (170970460 bp) 31921 reads (171455176 bp) 32001 reads (171949485 bp) 32087 reads (172438282 bp) 32210 reads (172923320 bp) 32297 reads (173407926 bp) 32378 reads (173897887 bp) 32463 reads (174385119 bp) 32551 reads (174877898 bp) 32648 reads (175361983 bp) 32712 reads (175852635 bp) 32796 reads (176336505 bp) 32885 reads (176820551 bp) 32959 reads (177304870 bp) 33072 reads (177792664 bp) 33151 reads (178279103 bp) 33232 reads (178767174 bp) 33325 reads (179255367 bp) 33410 reads (179741018 bp) 33477 reads (180225737 bp) 33550 reads (180709427 bp) 33638 reads (181195169 bp) 33696 reads (181678787 bp) 33820 reads (182167268 bp) 33902 reads (182665773 bp) 34014 reads (183154109 bp) 34097 reads (183642580 bp) 34183 reads (184129159 bp) 34300 reads (184614934 bp) 34385 reads (185099720 bp) 34455 reads (185589090 bp) 34537 reads (186082481 bp) 34617 reads (186591131 bp) 34754 reads (187076989 bp) 34832 reads (187570409 bp) 34910 reads (188062214 bp) 35034 reads (188547556 bp) 35152 reads (189034626 bp) 35244 reads (189519927 bp) 35342 reads (190004816 bp) 35433 reads (190489244 bp) 35549 reads (190976438 bp) 35653 reads (191463770 bp) 35738 reads (191949319 bp) 35827 reads (192434667 bp) 35920 reads (192924757 bp) 36010 reads (193416508 bp) 36094 reads (193900157 bp) 36179 reads (194384550 bp) 36269 reads (194879896 bp) 36339 reads (195375211 bp) 36441 reads (195860595 bp) 36536 reads (196356508 bp) 36651 reads (196841362 bp) 36748 reads (197327684 bp) 36837 reads (197819663 bp) 36928 reads (198317357 bp) 37011 reads (198809677 bp) 37082 reads (199307360 bp) 37171 reads (199802959 bp) 37254 reads (200292674 bp) 37356 reads (200790848 bp) 37441 reads (201278932 bp) 37533 reads (201764582 bp) 37642 reads (202248964 bp) 37742 reads (202736834 bp) 37828 reads (203220532 bp) 37918 reads (203713533 bp) 38036 reads (204199257 bp) 38120 reads (204684450 bp) 38210 reads (205186889 bp) 38295 reads (205674659 bp) 38385 reads (206167447 bp) 38482 reads (206657196 bp) 38586 reads (207141550 bp) 38678 reads (207637949 bp) 38759 reads (208134358 bp) 38843 reads (208624679 bp) 38948 reads (209125263 bp) 39016 reads (209609578 bp) 39101 reads (210095783 bp) 39182 reads (210580180 bp) 39266 reads (211072044 bp) 39366 reads (211558379 bp) 39467 reads (212045569 bp) 39547 reads (212540324 bp) 39641 reads (213025959 bp) 39747 reads (213515295 bp) 39821 reads (214000455 bp) 39890 reads (214494780 bp) 39961 reads (214983238 bp) 40050 reads (215468786 bp) 40124 reads (215958123 bp) 40216 reads (216461244 bp) 40312 reads (216945527 bp) 40393 reads (217457545 bp) 40522 reads (217942598 bp) 40600 reads (218433207 bp) 40709 reads (218920061 bp) 40785 reads (219420413 bp) 40866 reads (219905483 bp) 40986 reads (220393300 bp) 41076 reads (220879254 bp) 41152 reads (221363010 bp) 41219 reads (221851668 bp) 41298 reads (222337956 bp) 41408 reads (222826455 bp) 41562 reads (223313322 bp) 41651 reads (223806036 bp) 41739 reads (224295167 bp) 41815 reads (224785404 bp) 41908 reads (225272604 bp) 42001 reads (225757849 bp) 42077 reads (226242595 bp) 42166 reads (226731865 bp) 42276 reads (227227034 bp) 42389 reads (227711085 bp) 42465 reads (228223061 bp) 42564 reads (228720385 bp) 42646 reads (229204203 bp) 42736 reads (229689264 bp) 42853 reads (230174257 bp) 42983 reads (230664418 bp) 43073 reads (231150764 bp) 43153 reads (231634654 bp) 43255 reads (232133494 bp) 43320 reads (232627028 bp) 43398 reads (233112886 bp) 43500 reads (233602412 bp) 43578 reads (234088308 bp) 43664 reads (234573346 bp) 43751 reads (235068726 bp) 43891 reads (235559226 bp) 43962 reads (236042902 bp) 44041 reads (236529231 bp) 44125 reads (237014405 bp) 44227 reads (237501287 bp) 44347 reads (237987064 bp) 44441 reads (238474171 bp) 44543 reads (238959452 bp) 44626 reads (239443071 bp) 44704 reads (239926754 bp) 44770 reads (240410593 bp) 44826 reads (240897020 bp) 44953 reads (241389252 bp) 45055 reads (241874129 bp) 45138 reads (242367178 bp) 45205 reads (242850979 bp) 45317 reads (243336170 bp) 45460 reads (243756674 bp) Filtering long reads target: 219381006 bp keeping 219382270 bp Outputting passed long reads Uploading filtered reads: colony27_barcode07_filtlong.reads Generating and saving report. Filtlong results saved.
Assemble long reads using the Flye assembler.
This app completed without errors in 20m 55s.
Objects
Created Object Name Type Description
colony27_barcode7_flye.contigs Assembly Assembled contigs
Summary
Flye results saved to: mt2:narrative_1717703395562//kb/module/work/tmp/flye_1efd0076-554c-4d3b-a726-1cb3f8f52128 Assembly saved to: mt2:narrative_1717703395562/colony27_barcode7_flye.contigs Assembled into 3 contigs. Avg Length: 1610007.0 bp. Contig Length Distribution (# of contigs -- min to max basepairs): 1 -- 39793.0 to 313540.1 bp 0 -- 313540.1 to 587287.2 bp 0 -- 587287.2 to 861034.2999999999 bp 0 -- 861034.2999999999 to 1134781.4 bp 0 -- 1134781.4 to 1408528.5 bp 0 -- 1408528.5 to 1682275.5999999999 bp 0 -- 1682275.5999999999 to 1956022.6999999997 bp 1 -- 1956022.6999999997 to 2229769.8 bp 0 -- 2229769.8 to 2503516.9 bp 1 -- 2503516.9 to 2777264.0 bp
Links
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/184688
  • flye_output.zip - Output file(s) generated by Flye
Runs the CheckM lineage workflow to assess the genome quality of isolates, single cells, or genome bins from metagenome assemblies through comparison to an existing database of genomes.
This app completed without errors in 5m 3s.
Links
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/184688
  • CheckM_summary_table.tsv.zip - TSV Summary Table from CheckM
  • full_output.zip - Full output of CheckM
  • plots.zip - Output plots from CheckM
Annotate Assembly and Re-annotate Genomes with Prokka annotation pipeline.
This app completed without errors in 2m 44s.
Objects
Created Object Name Type Description
colony27_barcode07_prokka_annotated_genome Genome Annotated Genome
Summary
Annotated Genome saved to: mt2:narrative_1717703395562/colony27_barcode07_prokka_annotated_genome Number of genes predicted: 4456 Number of protein coding genes: 4393 Number of genes with non-hypothetical function: 2621 Number of genes with EC-number: 1150 Number of genes with Seed Subsystem Ontology: 0 Average protein length: 333 aa.
Allows users to create an AssemblySet object.
This app completed without errors in 11s.
Objects
Created Object Name Type Description
Colony27_AssemblySet AssemblySet KButil_Build_AssemblySet
Summary
assembly objs in output set Colony27_AssemblySet: 1
Obtain objective taxonomic assignments for bacterial and archaeal genomes based on the Genome Taxonomy Database (GTDB)
This app completed without errors in 23m 34s.
Objects
Created Object Name Type Description
colony27_barcode07_flye.contigs Assembly Added GTDB lineage
Colony27_AssemblySet AssemblySet Added GTDB lineage
Links
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/184688
  • gtdbtk.backbone.bac120.classify.tree - gtdbtk.backbone.bac120.classify.tree - whole tree GTDB formatted Newick
  • gtdbtk.backbone.bac120.classify-ITOL.tree - gtdbtk.backbone.bac120.classify-ITOL.tree - whole tree ITOL formatted Newick
  • gtdbtk.bac120.classify.tree.1.tree - gtdbtk.bac120.classify.tree.1.tree - whole tree GTDB formatted Newick
  • gtdbtk.bac120.classify.tree.1-ITOL.tree - gtdbtk.bac120.classify.tree.1-ITOL.tree - whole tree ITOL formatted Newick
  • gtdbtk.backbone.bac120.classify-proximals.tree - gtdbtk.backbone.bac120.classify-proximals.tree - Newick
  • gtdbtk.backbone.bac120.classify-trimmed.tree - gtdbtk.backbone.bac120.classify-trimmed.tree - Newick
  • gtdbtk.backbone.bac120.classify-lineages.map - gtdbtk.backbone.bac120.classify-lineages.map - GTDB lineage
  • gtdbtk.backbone.bac120.classify-trimmed.tree-rectangle.PNG - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-rectangle.PDF - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-circle.PNG - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-circle.PDF - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-circle-ultrametric.PNG - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-circle-ultrametric.PDF - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.1-proximals.tree - gtdbtk.bac120.classify.tree.1-proximals.tree - Newick
  • gtdbtk.bac120.classify.tree.1-trimmed.tree - gtdbtk.bac120.classify.tree.1-trimmed.tree - Newick
  • gtdbtk.bac120.classify.tree.1-lineages.map - gtdbtk.bac120.classify.tree.1-lineages.map - GTDB lineage
  • gtdbtk.bac120.classify.tree.1-trimmed.tree-rectangle.PNG - gtdbtk.bac120.classify.tree.1-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.1-trimmed.tree-rectangle.PDF - gtdbtk.bac120.classify.tree.1-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.1-trimmed.tree-circle.PNG - gtdbtk.bac120.classify.tree.1-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.1-trimmed.tree-circle.PDF - gtdbtk.bac120.classify.tree.1-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.1-trimmed.tree-circle-ultrametric.PNG - gtdbtk.bac120.classify.tree.1-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.1-trimmed.tree-circle-ultrametric.PDF - gtdbtk.bac120.classify.tree.1-trimmed.tree - Image
  • GTDB-Tk_classify_wf.zip - GTDB-Tk Classify WF output
Add one or more Genomes to a KBase SpeciesTree.
This app completed without errors in 4m 25s.
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/184688
  • colony27_barcode07_tree.newick
  • colony27_barcode07_tree-labels.newick
  • colony27_barcode07_tree.png
  • colony27_barcode07_tree.pdf

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    • Pierre-Alain Chaumeil, Aaron J Mussig, Philip Hugenholtz, Donovan H Parks, GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database, Bioinformatics, Volume 36, Issue 6, 15 March 2020, Pages 1925 1927. DOI: https://doi.org/10.1093/bioinformatics/btz848
    • Donovan H Parks, Maria Chuvochina, Christian Rinke, Aaron J Mussig, Pierre-Alain Chaumeil, Philip Hugenholtz. GTDB: an ongoing census of bacterial and archaeal diversity through a phylogenetically consistent, rank normalized and complete genome-based taxonomy. Nucleic Acids Research, Volume 50, Issue D1, 7 January 2022, Pages D785 D794. DOI: https://doi.org/10.1093/nar/gkab776
    • Parks, D., Chuvochina, M., Waite, D. et al. A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life. Nat Biotechnol 36, 996 1004 (2018). DOI: https://doi.org/10.1038/nbt.4229
    • Parks DH, Chuvochina M, Chaumeil PA, Rinke C, Mussig AJ, Hugenholtz P. A complete domain-to-species taxonomy for Bacteria and Archaea. Nat Biotechnol. 2020;10.1038/s41587-020-0501-8. DOI:10.1038/s41587-020-0501-8
    • Rinke C, Chuvochina M, Mussig AJ, Chaumeil PA, Dav n AA, Waite DW, Whitman WB, Parks DH, and Hugenholtz P. A standardized archaeal taxonomy for the Genome Taxonomy Database. Nat Microbiol. 2021 Jul;6(7):946-959. DOI:10.1038/s41564-021-00918-8
    • Chivian D, Jungbluth SP, Dehal PS, Wood-Charlson EM, Canon RS, Allen BH, Clark MM, Gu T, Land ML, Price GA, Riehl WJ, Sneddon MW, Sutormin R, Zhang Q, Cottingham RW, Henry CS, Arkin AP. Metagenome-assembled genome extraction and analysis from microbiomes using KBase. Nat Protoc. 2023 Jan;18(1):208-238. doi: 10.1038/s41596-022-00747-x
    • Matsen FA, Kodner RB, Armbrust EV. pplacer: linear time maximum-likelihood and Bayesian phylogenetic placement of sequences onto a fixed reference tree. BMC Bioinformatics. 2010;11:538. Published 2010 Oct 30. doi:10.1186/1471-2105-11-538
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    • Price MN, Dehal PS, Arkin AP. FastTree 2--approximately maximum-likelihood trees for large alignments. PLoS One. 2010;5(3):e9490. Published 2010 Mar 10. DOI:10.1371/journal.pone.0009490 link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835736/
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Apps in Beta

  1. Annotate Assembly and Re-annotate Genomes with Prokka - v1.14.5
    • Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30: 2068 2069. doi:10.1093/bioinformatics/btu153
  2. Assemble Long Reads with Flye - v2.9.4
    • [1] Mikhail Kolmogorov, Jeffrey Yuan, Yu Lin and Pavel Pevzner, "Assembly of Long Error-Prone Reads Using Repeat Graphs", Nature Biotechnology, 2019 doi:10.1038/s41587-019-0072-8
  3. Filter Reads with Filtlong - v0.2.1
    • [1] Wick RR, 2017. Filtlong.
  4. Insert Genome Into SpeciesTree - v2.2.0
    • Price MN, Dehal PS, Arkin AP. FastTree 2 Approximately Maximum-Likelihood Trees for Large Alignments. PLoS One. 2010;5. doi:10.1371/journal.pone.0009490