Generated July 19, 2024

Luteolibacter sp. strain Populi

Luteolibacter sp. strain Populi is bacterium from the phylum Verrucomicrobiota, isolated from the rhizosphere of a black cottonwood tree, Populus trichocarpa, from the Cascade mountains in Washington. Its 6.6 Mb chromosome was completely sequenced using Oxford Nanopore long-reads and is predicted to encode 5301 proteins and 60 RNAs.

The bacteria was isolated from the rhizosphere of a mature Populus trichocarpa from the Tieton riverwatershed of Washington state, USA (Lat: 46°42’9” N, Lon: 120°25 39’36” W). A rhizosphere sample (fine roots and adhering soil) was used to obtain a microbial fraction by centrifugation on Histodenz (12) and stained with 5µM Syto59 (Thermo Fisher Scientific Inc). A Cytopeia Influx cell sorter (BD, Franklin Lakes, NJ) was used to sort and array single cells (100 per plate) based on forward-side scatter and fluorescence intensity on asparagine-glucose nutrient agar (ATCC medium 184).

The Luteolibacter sp. Populi genome sequence has been deposited in GenBank under the accession number CP161812. A draft genome annotated with Prokka and DRAM is available in this Narrative as Luteolibacter_sp_Prokka.240711.

v3 - KBaseGenomes.Genome-11.1
The viewer for the data in this Cell is available at the original Narrative here: https://narrative.kbase.us/narrative/185119
from biokbase.narrative.jobs.appmanager import AppManager
AppManager().run_app_batch(
    [{
        "app_id": "kb_uploadmethods/import_fastq_noninterleaved_as_reads_from_staging",
        "tag": "release",
        "version": "5b9346463df88a422ff5d4f4cba421679f63c73f",
        "params": [{
            "fastq_fwd_staging_file_name": "bc07.fastq.gz",
            "fastq_rev_staging_file_name": None,
            "name": "Reads"
        }],
        "shared_params": {
            "sequencing_tech": "NanoPore",
            "single_genome": 1,
            "read_orientation_outward": 0,
            "insert_size_std_dev": None,
            "insert_size_mean": None
        }
    }],
    cell_id="8aeeac6b-770a-439e-9707-65f702cb1c5e",
    run_id="e5d964b1-293a-4171-a303-105164e7515f"
)
v1 - KBaseFile.SingleEndLibrary-2.1
The viewer for the data in this Cell is available at the original Narrative here: https://narrative.kbase.us/narrative/185119
Filter long reads using Filtlong.
This app completed without errors in 7m 1s.
Objects
Created Object Name Type Description
Reads_filtlong.240702 SingleEndLibrary Filtered reads
Summary
Getting long reads (from reads library object). command: filtlong --min_length 1000 --keep_percent 90 --target_bases 500000000 /kb/module/work/tmp/de62e53f-5274-4666-b282-48eb78210568.single.fastq > /kb/module/work/tmp/filtlong_output_ce1c3cb1-c6ca-415a-8013-826fa3cbccbc.fastq Scoring long reads 187 reads (483953 bp) 283 reads (968842 bp) 395 reads (1456544 bp) 492 reads (1942150 bp) 595 reads (2430973 bp) 685 reads (2916335 bp) 778 reads (3402774 bp) 864 reads (3894816 bp) 963 reads (4382397 bp) 1059 reads (4876335 bp) 1143 reads (5367208 bp) 1237 reads (5855299 bp) 1345 reads (6340449 bp) 1439 reads (6824961 bp) 1539 reads (7308946 bp) 1625 reads (7796921 bp) 1735 reads (8280871 bp) 1821 reads (8768929 bp) 1918 reads (9258324 bp) 2016 reads (9742366 bp) 2100 reads (10234033 bp) 2182 reads (10723759 bp) 2278 reads (11208994 bp) 2369 reads (11693278 bp) 2451 reads (12179175 bp) 2538 reads (12663535 bp) 2628 reads (13148645 bp) 2720 reads (13632471 bp) 2813 reads (14118981 bp) 2906 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bp) 631673 reads (3287021937 bp) 631759 reads (3287509069 bp) 631835 reads (3287995144 bp) 631922 reads (3288480307 bp) 632011 reads (3288966241 bp) 632099 reads (3289451266 bp) 632193 reads (3289936598 bp) 632282 reads (3290420713 bp) 632362 reads (3290911541 bp) 632453 reads (3291396746 bp) 632537 reads (3291885390 bp) 632618 reads (3292373499 bp) 632702 reads (3292862923 bp) 632797 reads (3293346938 bp) 632888 reads (3293836028 bp) 632975 reads (3294321189 bp) 633067 reads (3294807716 bp) 633166 reads (3295291988 bp) 633250 reads (3295777210 bp) 633336 reads (3296267563 bp) 633423 reads (3296759193 bp) 633525 reads (3297244676 bp) 633604 reads (3297733296 bp) 633704 reads (3298217160 bp) 633777 reads (3298704125 bp) 633859 reads (3299188488 bp) 633953 reads (3299672308 bp) 634052 reads (3300158039 bp) 634147 reads (3300662653 bp) 634241 reads (3301149516 bp) 634315 reads (3301644160 bp) 634402 reads (3302134731 bp) 634489 reads (3302624117 bp) 634589 reads (3303115100 bp) 634689 reads (3303604831 bp) 634779 reads (3304092423 bp) 634867 reads (3304593132 bp) 634960 reads (3305081858 bp) 635063 reads (3305566799 bp) 635159 reads (3306053265 bp) 635246 reads (3306548929 bp) 635338 reads (3307037905 bp) 635430 reads (3307527105 bp) 635505 reads (3308015261 bp) 635595 reads (3308500289 bp) 635669 reads (3308994533 bp) 635753 reads (3309478409 bp) 635857 reads (3309962748 bp) 635951 reads (3310448003 bp) 636045 reads (3310938746 bp) 636134 reads (3311422734 bp) 636213 reads (3311906499 bp) 636301 reads (3312391318 bp) 636398 reads (3312875032 bp) 636484 reads (3313372257 bp) 636584 reads (3313858937 bp) 636670 reads (3314343544 bp) 636760 reads (3314831817 bp) 636855 reads (3315325742 bp) 636942 reads (3315811081 bp) 637043 reads (3316300063 bp) 637145 reads (3316784085 bp) 637233 reads (3317270281 bp) 637322 reads (3317758614 bp) 637415 reads (3318251043 bp) 637509 reads (3318736080 bp) 637606 reads (3319223760 bp) 637696 reads (3319713870 bp) 637784 reads (3320201531 bp) 637870 reads (3320685998 bp) 637971 reads (3321171924 bp) 638048 reads (3321671180 bp) 638138 reads (3322155848 bp) 638228 reads (3322641830 bp) 638327 reads (3323133490 bp) 638413 reads (3323619179 bp) 638518 reads (3324120125 bp) 638613 reads (3324609237 bp) 638702 reads (3325095785 bp) 638782 reads (3325581566 bp) 638915 reads (3326070268 bp) 639014 reads (3326566048 bp) 639118 reads (3327050162 bp) 639196 reads (3327538054 bp) 639295 reads (3328023316 bp) 639397 reads (3328508896 bp) 639493 reads (3328999048 bp) 639579 reads (3329494130 bp) 639675 reads (3329978371 bp) 639764 reads (3330465288 bp) 639844 reads (3330954861 bp) 639934 reads (3331440600 bp) 640014 reads (3331925131 bp) 640117 reads (3332409091 bp) 640204 reads (3332895926 bp) 640297 reads (3333387571 bp) 640399 reads (3333873241 bp) 640486 reads (3334368336 bp) 640565 reads (3334854520 bp) 640660 reads (3335340254 bp) 640755 reads (3335831787 bp) 640836 reads (3336322956 bp) 640921 reads (3336810526 bp) 641009 reads (3337302899 bp) 641106 reads (3337786658 bp) 641195 reads (3338270832 bp) 641291 reads (3338756752 bp) 641388 reads (3339243191 bp) 641474 reads (3339727884 bp) 641569 reads (3340213321 bp) 641674 reads (3340702187 bp) 641786 reads (3341189938 bp) 641882 reads (3341676520 bp) 641970 reads (3342166771 bp) 642055 reads (3342651140 bp) 642147 reads (3343136417 bp) 642230 reads (3343620897 bp) 642325 reads (3344107262 bp) 642421 reads (3344591948 bp) 642519 reads (3345078782 bp) 642616 reads (3345566986 bp) 642714 reads (3346052661 bp) 642814 reads (3346537988 bp) 642917 reads (3347026230 bp) 643023 reads (3347515892 bp) 643111 reads (3348001050 bp) 643201 reads (3348488198 bp) 643287 reads (3348973974 bp) 643381 reads (3349464766 bp) 643476 reads (3349948981 bp) 643557 reads (3350433297 bp) 643640 reads (3350918485 bp) 643742 reads (3351403554 bp) 643841 reads (3351888860 bp) 643929 reads (3352373420 bp) 644011 reads (3352863038 bp) 644105 reads (3353347499 bp) 644199 reads (3353838416 bp) 644283 reads (3354331242 bp) 644371 reads (3354821258 bp) 644453 reads (3355312745 bp) 644546 reads (3355800027 bp) 644639 reads (3356286385 bp) 644743 reads (3356783904 bp) 644830 reads (3357279559 bp) 644924 reads (3357764371 bp) 645029 reads (3358248700 bp) 645122 reads (3358738134 bp) 645214 reads (3359223014 bp) 645303 reads (3359711967 bp) 645389 reads (3360197995 bp) 645484 reads (3360682299 bp) 645579 reads (3361168014 bp) 645674 reads (3361658796 bp) 645777 reads (3362147535 bp) 645867 reads (3362633262 bp) 645972 reads (3363129354 bp) 646064 reads (3363617522 bp) 646149 reads (3364106793 bp) 646234 reads (3364595670 bp) 646324 reads (3365079497 bp) 646419 reads (3365565032 bp) 646513 reads (3366049463 bp) 646609 reads (3366537616 bp) 646704 reads (3367023331 bp) 646785 reads (3367521736 bp) 646870 reads (3368010646 bp) 646959 reads (3368499780 bp) 647055 reads (3368988400 bp) 647139 reads (3369473096 bp) 647228 reads (3369961707 bp) 647317 reads (3370450684 bp) 647406 reads (3370936285 bp) 647511 reads (3371419982 bp) 647594 reads (3371904649 bp) 647697 reads (3372394604 bp) 647788 reads (3372882294 bp) 647879 reads (3373368084 bp) 647957 reads (3373854102 bp) 648044 reads (3374341268 bp) 648138 reads (3374829571 bp) 648213 reads (3375316345 bp) 648303 reads (3375806848 bp) 648405 reads (3376291231 bp) 648501 reads (3376777610 bp) 648612 reads (3377268002 bp) 648695 reads (3377754409 bp) 648787 reads (3378240661 bp) 648882 reads (3378730414 bp) 648984 reads (3379218430 bp) 649077 reads (3379704399 bp) 649171 reads (3380188729 bp) 649262 reads (3380679864 bp) 649360 reads (3381163616 bp) 649455 reads (3381652118 bp) 649547 reads (3382139744 bp) 649650 reads (3382624223 bp) 649746 reads (3383110993 bp) 649837 reads (3383601325 bp) 649925 reads (3384088828 bp) 650027 reads (3384572720 bp) 650115 reads (3385056574 bp) 650214 reads (3385542491 bp) 650305 reads (3386031125 bp) 650396 reads (3386516620 bp) 650475 reads (3387008791 bp) 650557 reads (3387493359 bp) 650653 reads (3387978049 bp) 650744 reads (3388470146 bp) 650837 reads (3388953974 bp) 650933 reads (3389437734 bp) 651019 reads (3389928716 bp) 651108 reads (3390413456 bp) 651269 reads (3390899017 bp) 651383 reads (3391387567 bp) 651471 reads (3391873654 bp) 651568 reads (3392361283 bp) 651660 reads (3392849090 bp) 651744 reads (3393337029 bp) 651830 reads (3393833745 bp) 651930 reads (3394318132 bp) 652028 reads (3394804422 bp) 652126 reads (3395289290 bp) 652226 reads (3395779212 bp) 652275 reads (3396050605 bp) Filtering long reads target: 500000000 bp keeping 500010655 bp Outputting passed long reads Uploading filtered reads: Reads_filtlong.240702 Generating and saving report. Filtlong results saved.
Assemble long reads using the Flye assembler.
This app completed without errors in 46m 33s.
Objects
Created Object Name Type Description
Luteolibacter_sp_Flye.240702 Assembly Assembled contigs
Summary
Flye results saved to: allenbh:narrative_1719333290213//kb/module/work/tmp/flye_90822d96-baf0-485f-be73-a508547b9937 Assembly saved to: allenbh:narrative_1719333290213/Luteolibacter_sp_Flye.240702 Assembled into 1 contigs. Avg Length: 6638577.0 bp. Contig Length Distribution (# of contigs -- min to max basepairs): 0 -- 6638576.5 to 6638576.6 bp 0 -- 6638576.6 to 6638576.7 bp 0 -- 6638576.7 to 6638576.8 bp 0 -- 6638576.8 to 6638576.9 bp 0 -- 6638576.9 to 6638577.0 bp 1 -- 6638577.0 to 6638577.1 bp 0 -- 6638577.1 to 6638577.2 bp 0 -- 6638577.2 to 6638577.3 bp 0 -- 6638577.3 to 6638577.4 bp 0 -- 6638577.4 to 6638577.5 bp
Links
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/185119
  • flye_output.zip - Output file(s) generated by Flye
Run QUAST (QUality ASsessment Tool) on a set of Assemblies to assess their quality.
This app completed without errors in 1m 24s.
Summary
All statistics are based on contigs of size >= 500 bp, unless otherwise noted (e.g., "# contigs (>= 0 bp)" and "Total length (>= 0 bp)" include all contigs). Assembly Luteolibacter_sp_Flye.240702 Luteolibacter.fasta_assembly # contigs (>= 0 bp) 1 1 # contigs (>= 1000 bp) 1 1 # contigs (>= 10000 bp) 1 1 # contigs (>= 100000 bp) 1 1 # contigs (>= 1000000 bp) 1 1 Total length (>= 0 bp) 6638577 6638581 Total length (>= 1000 bp) 6638577 6638581 Total length (>= 10000 bp) 6638577 6638581 Total length (>= 100000 bp) 6638577 6638581 Total length (>= 1000000 bp) 6638577 6638581 # contigs 1 1 Largest contig 6638577 6638581 Total length 6638577 6638581 GC (%) 63.56 63.56 N50 6638577 6638581 N75 6638577 6638581 L50 1 1 L75 1 1 # N's per 100 kbp 0.00 0.00 # predicted genes (unique) 5665 5612 # predicted genes (>= 0 bp) 5701 + 0 part 5649 + 0 part # predicted genes (>= 300 bp) 4991 + 0 part 4949 + 0 part # predicted genes (>= 1500 bp) 1053 + 0 part 1052 + 0 part # predicted genes (>= 3000 bp) 224 + 0 part 230 + 0 part
Links
Annotate Assembly and Re-annotate Genomes with Prokka annotation pipeline.
This app completed without errors in 4m 13s.
Objects
Created Object Name Type Description
Luteolibacter_sp_Prokka.240711 Genome Annotated Genome
Summary
Annotated Genome saved to: allenbh:narrative_1719333290213/Luteolibacter_sp_Prokka.240711 Number of genes predicted: 5447 Number of protein coding genes: 5386 Number of genes with non-hypothetical function: 1877 Number of genes with EC-number: 898 Number of genes with Seed Subsystem Ontology: 0 Average protein length: 360 aa.
v1 - KBaseGenomes.Genome-11.1
The viewer for the data in this Cell is available at the original Narrative here: https://narrative.kbase.us/narrative/185119
Allows users to compute fast whole-genome Average Nucleotide Identity (ANI) estimation.
This app completed without errors in 3m 26s.
Links
v3 - KBaseSearch.GenomeSet-2.1
The viewer for the data in this Cell is available at the original Narrative here: https://narrative.kbase.us/narrative/185119
Add a user-provided GenomeSet to a KBase SpeciesTree.
This app completed without errors in 6m 21s.
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/185119
  • Selective_tree.newick
  • Selective_tree-labels.newick
  • Selective_tree.png
  • Selective_tree.pdf
Allows user to create a GenomeSet without specifying names
This app completed without errors in 21s.
Objects
Created Object Name Type Description
Luteolibacter_Genomeset GenomeSet KButil_Batch_Create_GenomeSet
Summary
genome objs in output set Luteolibacter_Genomeset: 10
v1 - KBaseSearch.GenomeSet-2.1
The viewer for the data in this Cell is available at the original Narrative here: https://narrative.kbase.us/narrative/185119
Add a user-provided GenomeSet to a KBase SpeciesTree.
This app completed without errors in 7m 16s.
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/185119
  • Selected_genomes_tree.240711.newick
  • Selected_genomes_tree.240711-labels.newick
  • Selected_genomes_tree.240711.png
  • Selected_genomes_tree.240711.pdf
Obtain objective taxonomic assignments for bacterial and archaeal genomes based on the Genome Taxonomy Database (GTDB)
This app completed without errors in 35m 11s.
Objects
Created Object Name Type Description
Luteolibacter_ambystomatis Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_arcticus Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_yonseiensis Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_sp_Prokka.240711 Genome Taxonomy and taxon_assignment updated with GTDB
Akkermansia_muciniphila Genome Taxonomy unchanged, taxon_assignment added GTDB
Haloferula_rosea Genome Taxonomy unchanged, taxon_assignment added GTDB
Haloferula_luteola Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_flavescens Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_marinus Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_pohnpeiensis Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_rhizosphaerae Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_sp._G-1-1-1 Genome Taxonomy unchanged, taxon_assignment added GTDB
Luteolibacter_Genomeset GenomeSet Taxonomy and taxon_assignment updated with GTDB
Links
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/185119
  • gtdbtk.backbone.bac120.classify.tree - gtdbtk.backbone.bac120.classify.tree - whole tree GTDB formatted Newick
  • gtdbtk.backbone.bac120.classify-ITOL.tree - gtdbtk.backbone.bac120.classify-ITOL.tree - whole tree ITOL formatted Newick
  • gtdbtk.bac120.classify.tree.8.tree - gtdbtk.bac120.classify.tree.8.tree - whole tree GTDB formatted Newick
  • gtdbtk.bac120.classify.tree.8-ITOL.tree - gtdbtk.bac120.classify.tree.8-ITOL.tree - whole tree ITOL formatted Newick
  • gtdbtk.backbone.bac120.classify-proximals.tree - gtdbtk.backbone.bac120.classify-proximals.tree - Newick
  • gtdbtk.backbone.bac120.classify-trimmed.tree - gtdbtk.backbone.bac120.classify-trimmed.tree - Newick
  • gtdbtk.backbone.bac120.classify-lineages.map - gtdbtk.backbone.bac120.classify-lineages.map - GTDB lineage
  • gtdbtk.backbone.bac120.classify-trimmed.tree-rectangle.PNG - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-rectangle.PDF - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-circle.PNG - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-circle.PDF - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-circle-ultrametric.PNG - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.backbone.bac120.classify-trimmed.tree-circle-ultrametric.PDF - gtdbtk.backbone.bac120.classify-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.8-proximals.tree - gtdbtk.bac120.classify.tree.8-proximals.tree - Newick
  • gtdbtk.bac120.classify.tree.8-trimmed.tree - gtdbtk.bac120.classify.tree.8-trimmed.tree - Newick
  • gtdbtk.bac120.classify.tree.8-lineages.map - gtdbtk.bac120.classify.tree.8-lineages.map - GTDB lineage
  • gtdbtk.bac120.classify.tree.8-trimmed.tree-rectangle.PNG - gtdbtk.bac120.classify.tree.8-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.8-trimmed.tree-rectangle.PDF - gtdbtk.bac120.classify.tree.8-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.8-trimmed.tree-circle.PNG - gtdbtk.bac120.classify.tree.8-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.8-trimmed.tree-circle.PDF - gtdbtk.bac120.classify.tree.8-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.8-trimmed.tree-circle-ultrametric.PNG - gtdbtk.bac120.classify.tree.8-trimmed.tree - Image
  • gtdbtk.bac120.classify.tree.8-trimmed.tree-circle-ultrametric.PDF - gtdbtk.bac120.classify.tree.8-trimmed.tree - Image
  • GTDB-Tk_classify_wf.zip - GTDB-Tk Classify WF output
Annotate MAGs with DRAM and distill resulting annotations to create an interactive functional summary per genome. Use for KBase genome objects.
This app completed without errors in 4h 10m 20s.
Summary
Here are the results from your DRAM run.
Links
Files
These are only available in the live Narrative: https://narrative.kbase.us/narrative/185119
  • annotations.tsv - DRAM annotations in a tab separate table format
  • genes.faa - Genes as amino acids predicted by DRAM with brief annotations
  • product.tsv - DRAM product in tabular format
  • metabolism_summary.xlsx - DRAM metabolism summary tables
  • genome_stats.tsv - DRAM genome statistics table
Import a GFF3 and FASTA file from your staging area into your Narrative as a Genome data object
This app completed without errors in 1m 41s.
Objects
Created Object Name Type Description
Luteolibacter_flavescens Genome Imported Genome
Links
Import a GFF3 and FASTA file from your staging area into your Narrative as a Genome data object
This app completed without errors in 1m 46s.
Objects
Created Object Name Type Description
Luteolibacter_arcticus Genome Imported Genome
Links
Import a GFF3 and FASTA file from your staging area into your Narrative as a Genome data object
This app completed without errors in 1m 22s.
Objects
Created Object Name Type Description
Luteolibacter_marinus Genome Imported Genome
Links
Import a GFF3 and FASTA file from your staging area into your Narrative as a Genome data object
This app completed without errors in 1m 43s.
Objects
Created Object Name Type Description
Luteolibacter_pohnpeiensis Genome Imported Genome
Links
Import a GFF3 and FASTA file from your staging area into your Narrative as a Genome data object
This app completed without errors in 1m 47s.
Objects
Created Object Name Type Description
Luteolibacter_rhizosphaerae Genome Imported Genome
Links
Import a GFF3 and FASTA file from your staging area into your Narrative as a Genome data object
This app completed without errors in 1m 26s.
Objects
Created Object Name Type Description
Luteolibacter_yonseiensis Genome Imported Genome
Links
Import a GFF3 and FASTA file from your staging area into your Narrative as a Genome data object
This app completed without errors in 1m 22s.
Objects
Created Object Name Type Description
Luteolibacter_ambystomatis Genome Imported Genome
Links
v1 - KBaseGenomes.Genome-11.0
The viewer for the data in this Cell is available at the original Narrative here: https://narrative.kbase.us/narrative/185119

Apps

  1. Annotate and Distill Genomes with DRAM
    • DRAM source code
    • DRAM documentation
    • DRAM Tutorial
    • DRAM publication
  2. Annotate Assembly and Re-annotate Genomes with Prokka - v1.14.5
    • Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30: 2068 2069. doi:10.1093/bioinformatics/btu153
  3. Assemble Long Reads with Flye - v2.9.4
    • [1] Mikhail Kolmogorov, Jeffrey Yuan, Yu Lin and Pavel Pevzner, "Assembly of Long Error-Prone Reads Using Repeat Graphs", Nature Biotechnology, 2019 doi:10.1038/s41587-019-0072-8
  4. Assess Quality of Assemblies with QUAST - v4.4
    • [1] Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics. 2013;29: 1072 1075. doi:10.1093/bioinformatics/btt086
    • [2] Mikheenko A, Valin G, Prjibelski A, Saveliev V, Gurevich A. Icarus: visualizer for de novo assembly evaluation. Bioinformatics. 2016;32: 3321 3323. doi:10.1093/bioinformatics/btw379
  5. Batch Create Genome Set - v1.2.0
    • Chivian D, Jungbluth SP, Dehal PS, Wood-Charlson EM, Canon RS, Allen BH, Clark MM, Gu T, Land ML, Price GA, Riehl WJ, Sneddon MW, Sutormin R, Zhang Q, Cottingham RW, Henry CS, Arkin AP. Metagenome-assembled genome extraction and analysis from microbiomes using KBase. Nat Protoc. 2023 Jan;18(1):208-238. doi: 10.1038/s41596-022-00747-x
    • Arkin AP, Cottingham RW, Henry CS, Harris NL, Stevens RL, Maslov S, et al. KBase: The United States Department of Energy Systems Biology Knowledgebase. Nature Biotechnology. 2018;36: 566. doi: 10.1038/nbt.4163
  6. Classify Microbes with GTDB-Tk - v2.3.2
    • Pierre-Alain Chaumeil, Aaron J Mussig, Philip Hugenholtz, Donovan H Parks. GTDB-Tk v2: memory friendly classification with the genome taxonomy database. Bioinformatics, Volume 38, Issue 23, 1 December 2022, Pages 5315 5316. DOI: https://doi.org/10.1093/bioinformatics/btac672
    • Pierre-Alain Chaumeil, Aaron J Mussig, Philip Hugenholtz, Donovan H Parks, GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database, Bioinformatics, Volume 36, Issue 6, 15 March 2020, Pages 1925 1927. DOI: https://doi.org/10.1093/bioinformatics/btz848
    • Donovan H Parks, Maria Chuvochina, Christian Rinke, Aaron J Mussig, Pierre-Alain Chaumeil, Philip Hugenholtz. GTDB: an ongoing census of bacterial and archaeal diversity through a phylogenetically consistent, rank normalized and complete genome-based taxonomy. Nucleic Acids Research, Volume 50, Issue D1, 7 January 2022, Pages D785 D794. DOI: https://doi.org/10.1093/nar/gkab776
    • Parks, D., Chuvochina, M., Waite, D. et al. A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life. Nat Biotechnol 36, 996 1004 (2018). DOI: https://doi.org/10.1038/nbt.4229
    • Parks DH, Chuvochina M, Chaumeil PA, Rinke C, Mussig AJ, Hugenholtz P. A complete domain-to-species taxonomy for Bacteria and Archaea. Nat Biotechnol. 2020;10.1038/s41587-020-0501-8. DOI:10.1038/s41587-020-0501-8
    • Rinke C, Chuvochina M, Mussig AJ, Chaumeil PA, Dav n AA, Waite DW, Whitman WB, Parks DH, and Hugenholtz P. A standardized archaeal taxonomy for the Genome Taxonomy Database. Nat Microbiol. 2021 Jul;6(7):946-959. DOI:10.1038/s41564-021-00918-8
    • Chivian D, Jungbluth SP, Dehal PS, Wood-Charlson EM, Canon RS, Allen BH, Clark MM, Gu T, Land ML, Price GA, Riehl WJ, Sneddon MW, Sutormin R, Zhang Q, Cottingham RW, Henry CS, Arkin AP. Metagenome-assembled genome extraction and analysis from microbiomes using KBase. Nat Protoc. 2023 Jan;18(1):208-238. doi: 10.1038/s41596-022-00747-x
    • Matsen FA, Kodner RB, Armbrust EV. pplacer: linear time maximum-likelihood and Bayesian phylogenetic placement of sequences onto a fixed reference tree. BMC Bioinformatics. 2010;11:538. Published 2010 Oct 30. doi:10.1186/1471-2105-11-538
    • Jain C, Rodriguez-R LM, Phillippy AM, Konstantinidis KT, Aluru S. High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nat Commun. 2018;9(1):5114. Published 2018 Nov 30. DOI:10.1038/s41467-018-07641-9
    • Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics. 2010;11:119. Published 2010 Mar 8. DOI:10.1186/1471-2105-11-119
    • Price MN, Dehal PS, Arkin AP. FastTree 2--approximately maximum-likelihood trees for large alignments. PLoS One. 2010;5(3):e9490. Published 2010 Mar 10. DOI:10.1371/journal.pone.0009490 link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2835736/
    • Eddy SR. Accelerated Profile HMM Searches. PLoS Comput Biol. 2011;7(10):e1002195. DOI:10.1371/journal.pcbi.1002195
    • Ondov BD, Treangen TJ, Melsted P, Mallonee AB, Bergman NH, Koren S, Phillippy AM. Mash: fast genome and metagenome distance estimation using MinHash. Genome Biol. 2016 Jun 20;17(1):132. DOI: 10.1186/s13059-016-0997-x
  7. Compute ANI with FastANI
    • [1] Jain C, Rodriguez-R LM, Phillippy AM, Konstantinidis KT, Aluru S. High-throughput ANI Analysis of 90K Prokaryotic Genomes Reveals Clear Species Boundaries. 2017; doi:10.1101/225342
    • [2] Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P, Tiedje JM. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol. 2007;57: 81 91. doi:10.1099/ijs.0.64483-0
    • FastANI module and source code:
  8. Filter Reads with Filtlong - v0.2.1
    • [1] Wick RR, 2017. Filtlong.
  9. Import GFF3/FASTA file as Genome from Staging Area
    no citations
  10. Insert Set of Genomes Into SpeciesTree - v2.2.0
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