Protocol for Genome Assembly and Annotation in KBase

A protocol paper in Current Protocols in Microbiology demonstrates an example workflow for taking a comparative and iterative approach to assembly and annotation of prokaryotic genomes using KBase. This approach can be used by microbiologists seeking to perform isolate analysis in a rapid and reproducible fashion. There is also a tutorial Narrative in KBase ( that replicates the workflow described in the protocol. KBase users can copy this Narrative to run the workflow on the included example reads or their own prokaryotic sequencing reads.

Allen B, Drake M, Harris N, Sullivan T. Using KBase to Assemble and Annotate Prokaryotic Genomes. Current Protocols in Microbiology. Hoboken, NJ, USA: John Wiley & Sons, Inc.; 2017. p. 1E.13.1-1E.13.18. doi:10.1002/cpmc.37 — Link to Publication