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Create Differential Expression Matrix using Ballgown - v3.5
kb_ballgown

v.2.1.0

Launch

Create differential expression matrix based on a given threshold cutoff (for eukaryotes only)

This App allows users to select the single-end or paired-end read files and provide the metadata for a set of samples to create an RNASeqSampleSet object. Note that the user must provide reads for two or more samples.

Users need to import single-end or paired-end read files to their Narrative before starting this App.

It is mandatory to select the "Library Type", "Domain", "RNA-seq Sample and Condition Labels" and provide the "RNA-seq Sample Set" (RNASeqSampleSet object name). All replicates for the same condition must have same label.

Users can optionally fill in additional metadata information in the advanced options.

Note: Ballgown requires at least one intron to function. Also, this app will not work on prokaryotes.

Team members who developed & deployed algorithm in KBase: Srividya Ramakrishnan, Sunita Kumari, Shinjae Yoo, Priya Ranjan, Jim Thomason, Vivek Kumar, Arfath Pasha. For questions, please contact us.


App Specification:

https://github.com/kbaseapps/kb_ballgown.git/tree/93af4c84c346641fd1b3cfa53f26856df07ade83/ui/narrative/methods/run_ballgown_app

Module Commit: 93af4c84c346641fd1b3cfa53f26856df07ade83